Effects of acute hypoxia and adenosine on splenic T-lymphocyte proliferation of rats / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To observe the effects of acute hypoxia and adenosine on splenic T lymphocyte proliferation.</p><p><b>METHODS</b>Wistar rats were divided into control and hypoxic group, and the latter were exposed to hypoxia (5000 m simulated high altitude, 23 h/d). Three days later, spleen cells were collected and stimulated by 5.0 microg/ml and 2.5 microg/ml concanavalin A (ConA) to determine the splenocyte proliferation. The proliferation was also observed after addition of different amount of adenosine to culture medium.</p><p><b>RESULTS</b>Acute hypoxia and adenosine had marked inhibitory effect on mitogenic response to Con A in splenic T cells, and the inhibitory effect induced by adenosine displayed concentration-dependent.</p><p><b>CONCLUSION</b>Acute hypoxia may impair the T cell function and adenosine could be involved in this process.</p>