Molecular identification of hairy antler by analysis of high resolution melting / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 619-623, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-330189
ABSTRACT
High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Chifres de Veado
/
Padrões de Referência
/
DNA
/
Cervos
/
Química
/
Reação em Cadeia da Polimerase
/
Classificação
/
Primers do DNA
/
Temperatura de Transição
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Artigo
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