Cloning and expression analysis of a acetyl-CoA U-acetyltransferase gene (TwAACT) from Tripterygium wilfordii / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 847-852, 2015.
Article
em Zh
| WPRIM
| ID: wpr-330222
Biblioteca responsável:
WPRO
ABSTRACT
In this study, based on the transcriptome data, we cloned the full-length cDNAs of TwAACT gene from Tripterygium wilfordii suspension cells, and then analyzed the bioinformation of the sequence, detected the genetic differential expression after being induced by methyl jasmonate (MeJA) by RT-PCR. The full-length cDNA of the TwAACT was 1 704 bp containing a 1 218 bp open reading frame (ORF) encoding a polypeptide of 405 amino acids (GeneBank accession No. KP297934). The deduced isoelectric point (pI) was 6.10, a calculated molecular weight was about 41.20 kDa, and online prediction showed that TwAACT had two catalytic active sites. After the induction of MeJA, the relative expression level of TwAACT increased rapidly. The expression level of TwAACT was highest at 24 h. TwAACT was cloned firstly, that laid the foundation for identifying thegene and illustrating thebiosynthesis mechanism and its synthetic biology.
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Índice:
WPRIM
Assunto principal:
Acetil-CoA C-Acetiltransferase
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Filogenia
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Proteínas de Plantas
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Dados de Sequência Molecular
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Modelos Moleculares
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Química
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Alinhamento de Sequência
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Sequência de Aminoácidos
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Classificação
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Clonagem Molecular
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Article