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Icariin promote maturation of osteoblasts in vitro by an estrogen-independent mechanism / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2704-2709, 2014.
Artigo em Chinês | WPRIM | ID: wpr-330266
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the estrogenic activity of icariin and genistein with estrogen-dependent human breast cancer (MCF-7) cells.</p><p><b>METHOD</b>MCF-7 cells were incubated with media containing 5% charcoal dextran-treated FBS in phenol red-free media for 48 h. CCK-8 kit was used to study the impact of defferent concentration of icariin and genistein on MCF-7 proliferation in vitro. Optimal concentration icariin and genistein were added into medium and total RNA was isolated after 12, 24, 36, 48 h. The gene expression of ERalpha, ERbeta, PS2, and PR were investigated by Real-time RT-PCR Total protein was also isolated and secretion of ERalpha, ERbeta, PS2, and PR were examined by Western blot.</p><p><b>RESULT</b>10 micromol x L(-1) icariin and genistein could promote the proliferation of MCF-7 evidently. However, the ability of genistein to promote the proliferation was better than icariin. With the concentration of 10 micromol x L(-1), genistein group had a stronger expression of ERa, PS2 and PR mRNA levels than icariin while ERbetaexpression had no significant difference in two group. The same effects were detected by western blotting.</p><p><b>CONCLUSION</b>Both genistein and icariin have a strong estrogen-like effect, but the estrogenic activity of genistein is stronger than icariin. It showed that the activity of icariin is stron-ger than genistein to promote ROB maturation. So it must be that icariin promotes the maturation of osteoblasts in vitro by a estogen-independent mechanism.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacologia / Flavonoides / Regulação da Expressão Gênica / Genisteína / Biologia Celular / Receptor alfa de Estrogênio / Receptor beta de Estrogênio / Proliferação de Células / Estrogênios Limite: Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacologia / Flavonoides / Regulação da Expressão Gênica / Genisteína / Biologia Celular / Receptor alfa de Estrogênio / Receptor beta de Estrogênio / Proliferação de Células / Estrogênios Limite: Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2014 Tipo de documento: Artigo