Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2208-2211, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-330319
ABSTRACT
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Escorpiões
/
Dados de Sequência Molecular
/
Contaminação de Medicamentos
/
Classificação
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Código de Barras de DNA Taxonômico
/
Proteínas de Artrópodes
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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