Identification of herbal tea ingredient Plumeria rubra and its adulterants using DNA barcoding / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2199-2203, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-330321
ABSTRACT
ITS2 sequence was used as a barcode to identify herbal tea ingredient Plumeria rubra and its adulterants. Genomic DNAs from forty eight samples were extracted, the ITS2 sequences were amplified and sequenced bi-direstionlly, and then assembled and obtained using CodonCode Aligner. The sequences were aligned using ClustalW, the genetic distances were computed by kimura 2-parameter (K2P) model and the Neighbor-joining (NJ) phylogenetic trees were constructed using MEGA5.0. Results showed that the length of ITS2 sequence of P. rubra were 244 bp. The intra-specific genetic distances (0-0. 016 6) were much smaller than inter-specific ones between P. rubra and its adulterants(0.320 8-0.650 4). The NJ tree indicated that P. rubra and its adulterants could be distinguished clearly. Therefore, Using ITS2 barcode can accurately andeffectively distinguish herbal tea ingredient P. rubra from its adulterants, which providesa new molecular method to identify P. rubra and ensure its safety in use.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Medicamentos de Ervas Chinesas
/
Dados de Sequência Molecular
/
Química
/
Contaminação de Medicamentos
/
Classificação
/
DNA de Plantas
/
DNA Espaçador Ribossômico
/
Apocynaceae
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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