Flow cytometric analysis for detecting mitochondrial permeability transition pore opening / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2097-2099, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-330773
ABSTRACT
<p><b>OBJECTIVE</b>To introduce a new method for detecting mitochondrial permeability transition pore (PTP) opening with flow cytometry using the resveratrol-inducing PTP opening model.</p><p><b>METHODS</b>Mitochondria were isolated from rat livers and selectively labeled with nonyl acridine orange. The mitochondrial membrane potential was detected using flow cytometry with TMRE (tetramethylrhodamine, ethyl ester) labeling. PTP opening induced by resveratrol was represented by the changes of mitochondrial side-scattering (SSC) detected by flow cytometry.</p><p><b>RESULTS</b>Flow cytometry was capable of defining the purity of the mitochondria isolated. The fluorescence intensities and SSC of the mitochondria were decreased after resveratrol treatment, indicating that resveratrol could induce PTP opening. Ciclosporin A inhibited resveratrol-induced PTP opening.</p><p><b>CONCLUSION</b>Flow cytometric analysis allows accurate and convenient detection of mitochondrial membrane potential, mitochondrial swelling and PTP opening.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rodaminas
/
Mitocôndrias Hepáticas
/
Apoptose
/
Proteínas de Transporte da Membrana Mitocondrial
/
Potencial da Membrana Mitocondrial
/
Citometria de Fluxo
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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