Optimization of lyophilization procedures for freeze-drying of human red blood cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2055-2058, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-330785
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the different parameters of the lyophilization procedures that affect the recovery of the rehydrated red blood cells (RBCs).</p><p><b>METHODS</b>Human RBCs loaded in tubes were cooled with 4 different modes and subjected to water bath at 25 degrees celsius;. The morphological changes of the RBCs were observed to assess the degree of vitrification, and the specimens were placed in the freeze-dryer with the temperature set up at 40, -50, -60, -70 and -80 degrees celsius;. The rates of temperature rise of the main and secondary drying in the lyophilization procedures were compared, and the water residue in the specimens was determined.</p><p><b>RESULTS</b>The protectant did not show ice crystal in the course of freezing and thawing. No significant difference was found in the recovery rate of the rehydrated RBCs freeze-dried at the minimum temperature of -70 degrees celsius; and -80 degrees celsius; (P > 0.05). The E procedure resulted in the maximum recovery of the RBCs (83.14% ± 9.55%) and Hb (85.33% ± 11.42%), showing significant differences from the other groups(P < 0.01 or 0.05). The recovery of the RBCs showed a positive correlation to the water residue in the samples.</p><p><b>CONCLUSION</b>Fast cooling in liquid nitrogen and shelf precooling at -70 degrees celsius; with a moderate rate of temperature rise in lyophylization and a start dry temperature close to the shelf equilibrium temperature produce optimal freeze-drying result of human RBCs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Preservação de Tecido
/
Biologia Celular
/
Eritrócitos
/
Liofilização
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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