Construction and identification of blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1939-1942, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-330798
ABSTRACT
<p><b>OBJECTIVE</b>To construct and identify blood type B antigen mimetic polypeptide-macrophage inflammatory protein 3beta (Mip3beta) double expression recombinant plasmid.</p><p><b>METHODS</b>The positive phage clone P1 was obtained using phage random 12-mer peptide library. Specific primers were designed to amplify the phage DNA of P1 and transmembrane domain and inner segment of PBluscript-Fas gene. The products of the amplification were linked into Mip3betav21 to construct blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid. The recombinant plasmid was transfected into human melanoma cell line B16 to identify its expression.</p><p><b>RESULTS AND CONCLUSION</b>Blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid is successfully obtained and expressed in human melanoma cell line B16.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Plasmídeos
/
Antígenos de Grupos Sanguíneos
/
Proteínas Recombinantes de Fusão
/
Proteínas Recombinantes
/
Expressão Gênica
/
Linhagem Celular Tumoral
/
Genética
/
Macrófagos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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