Dynamic observations of beta-catenin in chronic myeloid leukemia and its relationship with cytogenetic response / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1868-1873, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-330819
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in the expression of beta-catenin in patients with chronic myeloid leukemia (CML) in different phases, and explore the relationship between beta-catenin and the cytogenetic response to imatinib mesylate.</p><p><b>METHODS</b>Beta-catenin mRNA and protein expressions were detected by RT-PCR and Western blotting in the bone marrow mononuclear cells (BMMNCs) from 99 CML patients. The expressions of BCR-ABL fusion gene at both the mRNA and protein levels were detected by fluorescence in situ hybridization (FISH) in 94 patients before and during the one-year treatment with imatinib mesylate at the interval of 3 months, and the relationship between beta-catenin and cytogenetic response to imatinib mesylate was analyzed.</p><p><b>RESULTS</b>The expression of beta-catenin increased significantly in patients with blast crisis and accelerated phase (P<0.001), but showed no significant difference between normal subjects and CML patients in the chronic phase (P>0.05). The main cytogenetic remission rate was significantly higher in patients who were consistently negative for beta-catenin than in those consistently positive for beta-catenin or those with a positive transformation (P<0.001).</p><p><b>CONCLUSION</b>Beta-catenin overexpression in the progression of CML, consistent high level of beta-catenin or a positive transformation may indicate a poor response to imatinib, and early measures should be taken to increase the remission rate.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Piperazinas
/
Pirimidinas
/
Benzamidas
/
RNA Mensageiro
/
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Crise Blástica
/
Estudos de Casos e Controles
/
Usos Terapêuticos
/
Tratamento Farmacológico
Tipo de estudo:
Estudo observacional
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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