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Inhibitory effect of melatonin on the growth of H22 hepatocarcinoma cells by inducing apoptosis / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 19-31, 2004.
Artigo em Inglês | WPRIM | ID: wpr-330883
ABSTRACT
Whether melatonin not only inhibits the growth of H22 hepatocarcinoma cells but also induces apoptosis in vitro was assessed. The anti-proliferative effects of melatonin on tumor cells was observed by MTT assay and tumor cells growth curve assay. And the apoptosis of the cells was studied by acridine orange fluorescence assay and flow cytometry. The cell cycle of the tumor cells was also observed by flow cytometry. It was found that melatonin could significantly inhibit the growth of H22 hepatocarcinoma cells. Incubated with melatonin, chromatin condensation of the tumor cells was observed by fluorescence microscopy. Compared with control, the percentage of apoptotic cells was increased, and the proportion of G0/S increased but that of G2/M decreased. It was suggested that melatonin could directly inhibit the growth of H22 hepatocarcinoma cells by inducing apoptosis and extending the length of cell cycle of the tumor cells.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Células Tumorais Cultivadas / Ciclo Celular / Divisão Celular / Apoptose / Carcinoma Hepatocelular / Citometria de Fluxo / Neoplasias Hepáticas / Melatonina Limite: Humanos Idioma: Inglês Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Células Tumorais Cultivadas / Ciclo Celular / Divisão Celular / Apoptose / Carcinoma Hepatocelular / Citometria de Fluxo / Neoplasias Hepáticas / Melatonina Limite: Humanos Idioma: Inglês Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Ano de publicação: 2004 Tipo de documento: Artigo