SUDHL-4 cell culture in vitro and establishment of mouse tumor model / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 329-334, 2012.
Article
em Zh
| WPRIM
| ID: wpr-330964
Biblioteca responsável:
WPRO
ABSTRACT
This study was designed to investigate the biological and immunological characteristics of a human diffuse large B-cell lymphoma (DLBCL) cell line SUDHL-4, and to establish a mouse model for human DLBCL. SUDHL-4 cells were cultured under different conditions. The morphology and in vitro expression of B-cell and tumor-related markers were detected by microscopy and flow cytometry respectively. To establish the transplanted tumor, the cells were injected subcutaneously into SCID mice. Tumor formation and its histomorphology were analyzed. The results showed that the expression of B cell/tumor-related markers was found on cultured SUDHL-4 cells. A stable mouse model of human DLBCL was successfully established in SCID mice by subcutaneous injection of 10(7) SUDHL-4 cells. Tumor tissue from mice exhibited similar histologic manifestation to those of human DLBCL. It is concluded that the SUDHL-4 cells represent a high consistency in immunological characteristics with human DLBCL. Transplantation of SUDHL-4 cells provides a syngeneic mouse model for the study of human DLBCL.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Linfoma Difuso de Grandes Células B
/
Camundongos SCID
/
Linhagem Celular Tumoral
/
Modelos Animais de Doenças
/
Citometria de Fluxo
/
Neoplasias Experimentais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2012
Tipo de documento:
Article