Clinical significance of monitoring BK polyomavirus in patients after hematopoietic stem cell transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 142-145, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-331002
ABSTRACT
This study was aimed to establish a method for rapid detecting BK polyomavirus (BKV) and to investigate the feasibility and value used in leukemia patients undergoing hematopoietic stem cell transplantation. Primers were designed according to BKV gene sequence; the quantitative standards for BKV and a real-time fluorescent quantitative PCR for BKV were established. The BKV level in urine samples from 36 patients after hematopoietic stem cell transplantation were detected by established method. The results showed that the standard of reconstructed plasmid and real time fluorescent quantitative PCR method were successfully established, its good specificity, sensitivity and stability were confirmed by experiments. BKV was found in 55.56% of urine samples, and the BKV load in urine was 2.46 × 10(4) - 7.8 × 10(9) copy/ml. It is concluded that the establishment of real-time fluorescent quantitative PCR for BKV detection provides a method for early diagnosis of the patients with hemorrhagic cystitis after hematopoietic stem cell transplantation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Urina
/
Virologia
/
DNA Viral
/
Estudos de Casos e Controles
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Vírus BK
/
Transplante de Células-Tronco Hematopoéticas
/
Carga Viral
/
Cistite
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
/
Estudo observacional
/
Estudo de rastreamento
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2012
Tipo de documento:
Artigo
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