PTEN induces anoikis through its phosphatase activity in hepatocellular carcinoma cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 273-275, 2005.
Article
em Zh
| WPRIM
| ID: wpr-331173
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and mechanisms of tumor suppressor gene PTEN on the induction of anoikis of hepatocellular carcinoma SMMC-7721 cells.</p><p><b>METHODS</b>SMMC-7721 cells were transfected with GFP plasmids containing wild-type PTEN or phosphatase inactivating mutant PTEN (C124A-PTEN) in vitro; The PTEN expression and the phosphorylation levels of focal adhesion kinase (FAK) and protein kinase B (PKB/Akt) were detected by Western blotting; Flow cytometry assay and laser scanning confocal microscopy were used to analyze apoptosis in adherent and non-adherent cells.</p><p><b>RESULTS</b>Compared with the control, PTEN expression in the cells transfected with wild-type PTEN increased to 248%, while the phosphorylation level of FAK and Akt decreased 65.2% and 89.1%, respectively; and the anoikis percentage increased from 9.5% to 31.3%. In the cells transfected with C124A-PTEN, neither the phosphorylation of FAK and Akt nor the anoikis percentage had obviously changed, although the PTEN expression enhanced dramatically in comparison with the control.</p><p><b>CONCLUSION</b>Through its phosphatase activity, tumor suppressor gene PTEN can suppress the phosphorylation of FAK and Akt, and induce anoikis in hepatocellular carcinoma cells.</p>
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Índice:
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Assunto principal:
Patologia
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Fosforilação
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Fisiologia
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Células Tumorais Cultivadas
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Carcinoma Hepatocelular
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Monoéster Fosfórico Hidrolases
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Anoikis
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PTEN Fosfo-Hidrolase
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Proteína-Tirosina Quinases de Adesão Focal
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2005
Tipo de documento:
Article