Mechanism of apoptosis induced by specific COX-2 inhibitor SC236 in gastric cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 145-147, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-331206
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the underlying mechanism of apoptosis-inducing effect of a specific COX-2 inhibitor SC236 in gastric cancer cells.</p><p><b>METHODS</b>Western blot analysis was used to measure apoptosis-related proteins, cytochrome c, and caspase-3. The catalytic activity of the caspases was measured using a colorimetric assay.</p><p><b>RESULTS</b>Treatment of AGS gastric cancer cells with SC236 caused a significant elevation of the pro-apoptotic protein Bak, release of cytochrome c to the cytosol, and activation of caspase-3. A specific caspase-3 inhibitor, z-DEVD-fmk, blocked SC236-induced apoptosis.</p><p><b>CONCLUSION</b>SC236 inhibits cell growth and induces apoptosis in gastric cancer cells at least partly through the up-regulation of Bak, stimulation of cytochrome c release, and activation of caspase-3.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligopeptídeos
/
Patologia
/
Farmacologia
/
Pirazóis
/
Neoplasias Gástricas
/
Sulfonamidas
/
Adenocarcinoma
/
Poli(ADP-Ribose) Polimerases
/
Apoptose
/
Caspases
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2005
Tipo de documento:
Artigo
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