Screening of differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 138-140, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-331208
ABSTRACT
<p><b>OBJECTIVE</b>To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p><p><b>METHODS</b>A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastasis Hca-F and its synogenetic cell line Hca-P with low metastatic potential was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.</p><p><b>RESULTS</b>Ten differentially expressed cDNA fragments of Hca-F with high potential of lymphatic spreading were obtained, two of which were newly identified ones.</p><p><b>CONCLUSION</b>SSH is a useful technique to detect genes of differential expression and an effective method to clone novel genes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
RNA Mensageiro
/
Regulação Neoplásica da Expressão Gênica
/
Biblioteca Gênica
/
Carcinoma Hepatocelular
/
Perfilação da Expressão Gênica
/
Linhagem Celular Tumoral
/
Genética
/
Neoplasias Hepáticas Experimentais
/
Metástase Linfática
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2005
Tipo de documento:
Artigo
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