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Alpha,beta-poly(N-hydroxypropyl/aminoethyl)-DL-aspartamide -co-L-lysine: potential non-viral vehicle for gene delivery / 生物医学工程学杂志
Article em Zh | WPRIM | ID: wpr-331387
Biblioteca responsável: WPRO
ABSTRACT
A series of Poly[aspartic acid-co-L-lysine](PAL) are copolycondensed by DL-aspartice acid and L-lysine with different ratios. Their constructions are identified by the spectra of 1H-NMR, FT-IR, X-Ray). These spectra are proved to have good regularity of these copolymers. alpha,beta-Poly[(N-hydroxypropyl/aminoethyl)-DL-Aspartamide-co-L-lysine] (PHAAL) is synthesized by ring-opening poly [aspartic acid-co-lysine] (PAL). PHAAL has good degradability in the phosphoric acid buffer solution (0.01 M, pH = 7.4) in the enzyme solution (Papain, Trypsine). PHAAL appeared tobe low cytotoxicity in Hela, ECV-304, Bcap37 cell lines, which was quantified by MTT assay. The combination ability of PHAAL with plasmid DNA was evaluated by agarose gel electrophoresis with agarose gel (1.0% w/v) containing ethidium bromide (0.25 microg/ml). The PHAAL with higher ratios of lysine in the copolymers have higher ability of condensing DNA. In summary, PHAAL, the polyaminoacid materials, could be one kind of macromolecule materials tobeused as the non-viral gene vehicle.
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Cordão Umbilical / Biopolímeros / Teste de Materiais / Células HeLa / Terapia Genética / Química / Ácido Aspártico / Técnicas de Transferência de Genes / Biologia Celular / Células Endoteliais Limite: Humans Idioma: Zh Revista: Journal of Biomedical Engineering Ano de publicação: 2007 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Cordão Umbilical / Biopolímeros / Teste de Materiais / Células HeLa / Terapia Genética / Química / Ácido Aspártico / Técnicas de Transferência de Genes / Biologia Celular / Células Endoteliais Limite: Humans Idioma: Zh Revista: Journal of Biomedical Engineering Ano de publicação: 2007 Tipo de documento: Article