Construction of the recombinant integrating shuttle plasmid with cfpl0-esat6 fusion gene of Mycobacterium tuberculosis and its expression in BCG / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1298-1302, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-331426
ABSTRACT
This study was conducted to amplify the cfpl0-esat6 fusion gene by SOE and insert into the integrating shuttle plasmid pMV361 to form the recombinant plasmid. Then another recombinant plasmid was constructed by insertinga-A g signal sequence of BCG. The two recombinant plasmids were introduced into BCG and the induced products from recombinant BCG were analyzed. In conclusion,the successful construction of rBCG expressing the fusion protein CFP10-ESAT6 will be the base of the development of novel Mycobacterium tuberclosis vaccines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Proteínas de Bactérias
/
Proteínas Recombinantes de Fusão
/
Vacinas contra a Tuberculose
/
Alergia e Imunologia
/
Genética
/
Metabolismo
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
/
Antígenos de Bactérias
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2006
Tipo de documento:
Artigo
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