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Effects of Panax notoginseng on the transdifferentiation of fibroblasts in human hypertrophic scar in vitro / 中华烧伤杂志
Chinese Journal of Burns ; (6): 188-190, 2007.
Artigo em Chinês | WPRIM | ID: wpr-331500
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Panax notoginseng on the transdifferentiation of the cultured human fibroblasts from hypertrophic scar in vitro, and explore its anti-fibrosis mechanism.</p><p><b>METHODS</b>The fibroblasts from human hypertrophic scar were cultured in vitro. Different amount of Panax notoginseng was added into the medium, respectively (400 microg/ml and 800 microg/ml). A culture without addition of the drug served as control. The fibroblast-populated collagen lattice method was used to detect the gel contraction, and contraction ratio was calculated. The immunocytochemistry staining method was used to detect the expression of alpha-smooth muscle actin. The flow cytometry method was used to detect the positive rate of alpha-smooth muscle actin.</p><p><b>RESULTS</b>The contraction degree of the fibroblasts after PNS administration was ameliorated at each time-point, with contraction index lower than that of controls (P < 0.05 or P < 0.01). Scattered distribution of alpha-SMA positive granules were observed in the cytoplasma, and the positive rate of alpha-SMA expression in 400 microg/ml (31.52%) and 800 microg/ml (24.28%) PNS groups were obviously lower than that in control group (45.74%, P < 0.05). The staining intensity of positive cells in 400 microg/ml and 800 microg/ml PNS groups was also obviously lower than that in control group (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>Panax notoginseng can inhibit the transdifferentiation of the cultured human fibroblasts from hypertrophic scar, and it exhibits an anti-fibrosis effect on hypertrophic scar in vitro.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Cicatrização / Divisão Celular / Células Cultivadas / Química / Cicatriz Hipertrófica / Biologia Celular / Ginsenosídeos / Panax notoginseng Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Burns Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Cicatrização / Divisão Celular / Células Cultivadas / Química / Cicatriz Hipertrófica / Biologia Celular / Ginsenosídeos / Panax notoginseng Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Burns Ano de publicação: 2007 Tipo de documento: Artigo