Influence of adenovirus transfection on the maturation characteristics of human immature dendritic cells / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the changes in the phenotype characteristics and immune function after transfection of cord blood derived immature dendritic cells( imDC) with Adeasy-EGFP adenovirus vector, and to explore the function of IL-10 in inhibition of imDC maturation.</p><p><b>METHODS</b>Immature dendritic cells were generated from human cord blood(CB) monocyte cultured with rhGM-CSF and rhIL-4. The recombinant adenovirus vector AdEASY-EGFP was transduced into immature dendritic cells on the third day with or without adding IL-10. The expression of cell maturation marker CD83, CD86 and HLA-DR were determined with flow cytometry. Allogeneic mixed leukocyte reaction( MLR) was used to examine the imDC's ability to promote T cell proliferation.</p><p><b>RESULTS</b>The expression of surface maturation markers of imDC after transfection with adenovirus were significantly up-regulated ( CD8646+/-10; CD83 38 +/- 7; HLA-DR 82 + 12) , and its ability to promote T cell proliferation was also obviously increased( SI > 2. 0). However, the expression of surface maturation markers of imDC after IL-10 treatment had lower mature phenotypes expression after transduction (CD868 +/- 5; CD83 9 +/- 3; HLA-DR63 +/- 12), and T cell stimulating ability was decreased comparing with adenovirus transfection groups.</p><p><b>CONCLUSION</b>Adenovirus can be transduced into imDC with high efficiency, but transfection itself can promote imDC's maturation. IL-10 treatment can inhibit the tendency to maturation stimulated by adenovirus transduction efficiently.</p>