Generation and phenotype analysis of zebrafish mutations of obesity-related genes lepr and mc4r / 生理学报
Acta Physiologica Sinica
;
(6): 61-69, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-331592
ABSTRACT
Obesity has become a severe public health problem across the world, and seriously affects the health and life quality of human beings. Here we generated lepr and mc4r mutant zebrafish via the CRISPR/Cas9 technique, and performed morphological and functional characterizations of those mutants. We observed that there was no significant phenotypic difference between homozygous mutants and wild-type controls before 2.5 months post-fertilization (mpf). However, the adult leprand mc4rindividuals displayed increased food intake, heavier weight, and higher body fat percentage, the characteristics of obesity phenotypes. Blood glucose test showed that overfeeding induced significantly impaired glucose tolerance in adult leprand mc4rzebrafish. Furthermore, we analyzed 76 energy metabolism-related transcripts in leprand mc4rzebrafish livers by using real-time RT-PCR, and compared the results with the published microarray data of Lepmouse livers, and found that the changes in the expression of insulin/IGF signaling (IIS) pathway genes in leprzebrafish and Lepmouse were positively correlated, suggesting that the IIS pathway maintains functional conservation between zebrafish and mammals during the evolution of the obesity-regulating molecule network.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peixe-Zebra
/
Transdução de Sinais
/
Leptina
/
Proteínas de Peixe-Zebra
/
Receptor Tipo 4 de Melanocortina
/
Receptores para Leptina
/
Técnicas de Inativação de Genes
/
Sistemas CRISPR-Cas
/
Genética
/
Insulina
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2017
Tipo de documento:
Artigo
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