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Natriuretic peptide precursor C coding gene contributes to zebrafish angiogenesis / 生理学报
Acta Physiologica Sinica ; (6): 11-16, 2017.
Artigo em Chinês | WPRIM | ID: wpr-331598
ABSTRACT
This study aimed to investigate the expression of the natriuretic peptide precursor C coding gene nppc and its role in angiogenesis during embryonic period of the zebrafish. Whole mount in situ hybridization was performed to detect the expression pattern of nppc. nppc specific morpholino and nppc mRNA were injected respectively into the one-cell stage embryo to specifically knock-down and rescue the expression of nppc in Tg (flk1GFP) and Tg (fli1anGFP) transgenic lines. The morphology and endothelial cell number of intersegmental vessel (ISV) were analyzed after imaging using the laser scanning confocal microscope. The results revealed that nppc was expressed in the brain, heart and vasculature of zebrafish larvae at 24 and 48 hours post-fertilization (hpf). Knock-down of nppc affected the development of ISV. Endothelial cell number was reduced after the knock-down of nppc. These results suggest that nppc controls zebrafish angiogenesis by affecting the endothelial cell proliferation and migration.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Peixe-Zebra / RNA Mensageiro / Animais Geneticamente Modificados / Movimento Celular / Neovascularização Fisiológica / Proteínas de Peixe-Zebra / Células Endoteliais / Peptídeos Natriuréticos / Proliferação de Células Limite: Animais Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Peixe-Zebra / RNA Mensageiro / Animais Geneticamente Modificados / Movimento Celular / Neovascularização Fisiológica / Proteínas de Peixe-Zebra / Células Endoteliais / Peptídeos Natriuréticos / Proliferação de Células Limite: Animais Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2017 Tipo de documento: Artigo