Effect of sodium valproate on human myelodysplastic syndrome cell line SKM-1 and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1515-1519, 2010.
Article
em Zh
| WPRIM
| ID: wpr-332327
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the effect of sodium valproate(VPA) on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flipl, c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibited the growth of SKM-1 cells in dose- and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibited proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Síndromes Mielodisplásicas
/
RNA Mensageiro
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Ácido Valproico
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Apoptose
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Peptídeos e Proteínas de Sinalização Intercelular
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Linhagem Celular Tumoral
/
Proliferação de Células
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Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2010
Tipo de documento:
Article