Detection of minimal residual disease in childhood acute lymphoblastic leukemia by using real-time quantitative PCR / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1235-1239, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-332386
ABSTRACT
This study was purposed to detect the minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) by using real time quantitative PCR (RQ-PCR) . The Ig and TCR gene rearrangements were amplified by using 18 primer sets in B-ALL, 8 primer sets in T-ALL; the ALL-MRD levels were quantified by using RQ-PCR with SYBR green dye staining and clone specific Ig/TCR gene rearrangements as molecular markers. The results indicated that there were 8 cases showing gene rearrangements in 9 B-ALL patients, marker detection rate for all samples was 88.8%, the MRD level on day 33 during induction treatment decreased significantly. It is concluded that Ig/TCR gene rearrangements can be used as a marker to detect MRD in childhood ALL; the technique of QR-PCR with SYBR green dye staining is reliable, relatively sensitive and easy performable method which can be used in routine detection for childhood ALL.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rearranjo Gênico
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Neoplasia Residual
/
Genes Codificadores dos Receptores de Linfócitos T
/
Diagnóstico
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2010
Tipo de documento:
Artigo
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