Preparations of the specific antibodies against exon 2 and exon 3 of human tau protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 146-148, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-332400
ABSTRACT
<p><b>OBJECTIVE</b>To prepare the specific antibodies against exon 2 and exon 3 of human tau protein.</p><p><b>METHODS</b>Sequences encoding exon 2 and exon 3 of human tau protein were amplified from human peripheral blood DNA and cloned into a prokaryotic expression vector pGEX-2T. Fusion proteins GST-E2 and GST-E3 were expressed and purified from E. coli system. The antisera were elicited by immunization of the purified fusion proteins to rabbits and mice. The specific antibodies were purified by Protein G/A and CNBr-activated sepharose 4B coupled with GST protein. The specificity and sensitivity of the purified antibodies were evaluated by Western blotting and ELISA.</p><p><b>RESULTS</b>Recombinant fusion proteins GST-E2 and GST-E3 were expressed and purified from E. coli, which showed Mr. 29 x 10(3). Various antisera were collected from the immunized experimental animals. Reliable immunoreactive specificity and titers of the purified antibodies against exon 2 and exon 3 of human tau protein were confirmed by Western blotting and ELISA after serial purification processes.</p><p><b>CONCLUSION</b>Four specific antibodies against exon 2 and exon 3 of human tau protein have been successfully prepared, which provides basis for analyzing the role of tau in neurodegenerative diseases.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Expressão Gênica
/
Éxons
/
Proteínas tau
/
Alergia e Imunologia
/
Escherichia coli
/
Genética
/
Metabolismo
/
Camundongos Endogâmicos BALB C
/
Anticorpos
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2009
Tipo de documento:
Artigo
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