Genetic characterization of the N protein of subgroups A and B human respiratory syncytial viruses / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 115-117, 2009.
Article
em Zh
| WPRIM
| ID: wpr-332411
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.</p><p><b>METHODS</b>Reverse transciption polymerase chain reaction (RT-PCR) was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004. The RT-PCR products were sequenced for N protein coding region. The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.</p><p><b>RESULTS</b>The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36-40 (3.1%-3.4%) and 4 (1.0%). The differences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17 (1.4%) and 1 (0.3%). The differences in number of nucleotide and deduced amino acid were 3-172 (0.25%-14.63%) and 0-18 (0-4.6%) respectively between 4 Beijing 2004 isolates and GenBank sequences.</p><p><b>CONCLUSION</b>N gene is the highly conservative gene in the HRSV genome. The variation between A and B subgroups were widely distributed in the entire gene of N protein, while the variation within the A or B subgroups HRSV was considerably lower.</p>
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Índice:
WPRIM
Assunto principal:
Filogenia
/
Virologia
/
Dados de Sequência Molecular
/
Química
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Classificação
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Homologia de Sequência de Aminoácidos
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Vírus Sincicial Respiratório Humano
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Infecções por Vírus Respiratório Sincicial
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Proteínas do Nucleocapsídeo
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2009
Tipo de documento:
Article