Application of 293 cells in in vitro detection of carcinogens / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 47-49, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-332433
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study.</p><p><b>METHODS</b>The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells.</p><p><b>RESULTS</b>293 cell acted like tumor cells after induced by MC-LR serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice.</p><p><b>CONCLUSION</b>293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinógenos
/
Testes de Carcinogenicidade
/
Linhagem Celular
/
Transformação Celular Neoplásica
/
Camundongos SCID
/
Toxicidade
/
Microcistinas
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2009
Tipo de documento:
Artigo
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