Development of an amplified luminescent proximity homogeneous immunoassay kit for detecting human hepatitis B virus e antibody / 南方医科大学学报
Journal of Southern Medical University
;
(12): 810-812, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-332545
ABSTRACT
<p><b>OBJECTIVE</b>To develop an amplified luminescent proximity homogeneous immunoassay (AlphaLISA) kit for the detection of human hepatitis B virus e antibody (HBeAb).</p><p><b>METHODS</b>The neutralizing and competitive inhibition method was used to develop the AlphaLISA kit for detection of serum HBeAb.</p><p><b>RESULTS</b>The working range of the kit was 0.003-16 NCU/ml with a sensitivity up to 0.003 NCU/ml. The intra- and inter-assay coefficient of variation was 5.3% and 6.8%, respectively. The kit showed no cross-reaction with HBcAb, and comparison of the detection results with those of a commercially available Elecsys HBeAb kit (Roche) for 136 samples showed a correlation coefficient of 0.961.</p><p><b>CONCLUSION</b>The AlphaLISA kit for HBeAb detection meets the clinical requirements for detection HBeAb in human serum.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Sangue
/
Imunoensaio
/
Desenho de Equipamento
/
Anticorpos Anti-Hepatite B
/
Medições Luminescentes
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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