Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase / 南方医科大学学报
Journal of Southern Medical University
;
(12): 686-689, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-332574
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase.</p><p><b>METHODS</b>The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions.</p><p><b>RESULTS</b>Ubiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT.</p><p><b>CONCLUSIONS</b>UXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Replicação Viral
/
Produtos do Gene pol
/
Vírus da Hepatite B
/
Técnicas do Sistema de Duplo-Híbrido
/
Mapeamento de Interação de Proteínas
/
Metabolismo
/
Proteínas de Neoplasias
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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