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Effect of BCL11A gene on transcription of γ-globin gene / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 628-632, 2013.
Artigo em Chinês | WPRIM | ID: wpr-332723
ABSTRACT
This study was aimed to explore the effect of BCL11A gene on transcription of γ-globin gene in K562 cells. B-cell lymphoma/leukemia 11A (BCL11A) gene was silenced by small interfering RNA (siRNA) expression vectors in K562 cells (human erythroblastic leukemia cell line). Gamma-globin mRNA level in K562 cells was determined by RT-PCR. Association between the BCL11A gene and γ-globin gene transcription was explored by comparison of mRNA levels. The results indicated that the silence rate of the BCL11A gene in K562 cells by 4 siRNA expression vectors was 49.7%, 55.4%, 78.2%, and 84.1%, respectively. The siRNA expression vector with 84.1% silence rate was transfected into K562 cells, transcription level of γ-globin mRNA in K562 cells transfected with siRNA expression vector increased 2.4 times as compared with control K562 cells. It is concluded that level of γ-globin mRNA increases when the BCL11A gene is silenced. It indicates that the BCL11A gene may be a negative regulator for γ-globin gene expression.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transcrição Gênica / Proteínas Nucleares / Transfecção / Proteínas de Transporte / Regulação Leucêmica da Expressão Gênica / Genes Reguladores / Células K562 / RNA Interferente Pequeno / Interferência de RNA / Gama-Globinas Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transcrição Gênica / Proteínas Nucleares / Transfecção / Proteínas de Transporte / Regulação Leucêmica da Expressão Gênica / Genes Reguladores / Células K562 / RNA Interferente Pequeno / Interferência de RNA / Gama-Globinas Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo