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Detection of weak D antigen by flow cytometry / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 474-477, 2013.
Artigo em Chinês | WPRIM | ID: wpr-332755
ABSTRACT
Flow cytometry was previously applied for analysis of Rh(D) antigen density, therefore it was suggested that the flow cytometry may be used for routine detection of weak D positive phenotypes. This study was purposed to evaluate its practicability. Six weak D positive and 7 DEL individuals were detected by using saline, IAT and absorption/elution test from 2010 to 2011 years. By RHD genotyping, zygosity analysis and sequencing, 3 cases of weak D type 15, 3 cases of partial D type DVI-III and 7 cases of DEL carrying RHD1227A alleles were identified. Taking 2 normal Rh(D)-positive and 2 D-negative samples as controls, all the samples were tested by using flow cytometry, and the median fluorescence intensities were observed as well. The results indicated that all weak D type 15 and partial D type DVI samples were detected to be positive by flow cytometry, as compared with 2 Rh(D)-negative samples (P < 0.05). Seven 7 DEL samples were tested to be negative (P > 0.05), although one of 7 DEL was tested as "±" in IAT and strong positive in absorption/elution. The RHD zygosity analysis showed this DEL individual as RHD(+)/RHD(+) homozygote. It is concluded that the sensitivity of detecting D antigen by flow cytometry is similar to that of IAT, but lower than absorption/elution test. As for detecting weak D or partial D antigens, IAT is easier than flow cytometry; as for identifying DEL, the flow cytometry is not sensitive enough.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenótipo / Sistema do Grupo Sanguíneo Rh-Hr / Sangue / Doadores de Sangue / Tipagem e Reações Cruzadas Sanguíneas / Alelos / Alergia e Imunologia / Citometria de Fluxo / Genética / Genótipo Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Adulto / Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenótipo / Sistema do Grupo Sanguíneo Rh-Hr / Sangue / Doadores de Sangue / Tipagem e Reações Cruzadas Sanguíneas / Alelos / Alergia e Imunologia / Citometria de Fluxo / Genética / Genótipo Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Adulto / Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo