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Cloning of differentially expressed genes following lipopolysaccharide stimulation in human umbilical vein endothelial cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology ; (6): 107-113, 2003.
Artigo em Inglês | WPRIM | ID: wpr-332908
ABSTRACT
<p><b>OBJECTIVE</b>To clone the differentially expressed genes in human umbilical vein endothelial cells (HUVEC) stimulated by lipopolysaccharide (LPS).</p><p><b>METHODS</b>Two-directional (forward and backward) suppression subtractive hybridization (SSH) was performed on HUVEC cultured in either standard media or treated for 6 hours with LPS (100 ng/ml). To restrict the number of false-positive clones, colony dot hybridization was used to further verify the differentially expressed cDNA clones. Positive clones were sequenced.</p><p><b>RESULTS</b>These analyses have identified both novel and known genes whose expression is influenced by LPS. The known genes include a group related to proinflammatory events, a group related to cellular apoptosis and proliferation, a group related to protein synthesis and cytoskeletal rearrangment, and a group related to energy metabolism and signal transduction.</p><p><b>CONCLUSIONS</b>SSH is a powerful technique of high sensitivity for the detection of differential gene expression in HUVEC stimulated by LPS.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Veias Umbilicais / Endotélio Vascular / Expressão Gênica / Células Cultivadas / Lipopolissacarídeos / Reação em Cadeia da Polimerase / Clonagem Molecular / DNA Complementar / Genética Limite: Humanos Idioma: Inglês Revista: Chinese Journal of Traumatology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Veias Umbilicais / Endotélio Vascular / Expressão Gênica / Células Cultivadas / Lipopolissacarídeos / Reação em Cadeia da Polimerase / Clonagem Molecular / DNA Complementar / Genética Limite: Humanos Idioma: Inglês Revista: Chinese Journal of Traumatology Ano de publicação: 2003 Tipo de documento: Artigo