Cloning of differentially expressed genes following lipopolysaccharide stimulation in human umbilical vein endothelial cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 107-113, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-332908
ABSTRACT
<p><b>OBJECTIVE</b>To clone the differentially expressed genes in human umbilical vein endothelial cells (HUVEC) stimulated by lipopolysaccharide (LPS).</p><p><b>METHODS</b>Two-directional (forward and backward) suppression subtractive hybridization (SSH) was performed on HUVEC cultured in either standard media or treated for 6 hours with LPS (100 ng/ml). To restrict the number of false-positive clones, colony dot hybridization was used to further verify the differentially expressed cDNA clones. Positive clones were sequenced.</p><p><b>RESULTS</b>These analyses have identified both novel and known genes whose expression is influenced by LPS. The known genes include a group related to proinflammatory events, a group related to cellular apoptosis and proliferation, a group related to protein synthesis and cytoskeletal rearrangment, and a group related to energy metabolism and signal transduction.</p><p><b>CONCLUSIONS</b>SSH is a powerful technique of high sensitivity for the detection of differential gene expression in HUVEC stimulated by LPS.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Veias Umbilicais
/
Endotélio Vascular
/
Expressão Gênica
/
Células Cultivadas
/
Lipopolissacarídeos
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
/
DNA Complementar
/
Genética
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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