Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 3-7, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-332927
ABSTRACT
<p><b>OBJECTIVE</b>To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.</p><p><b>METHODS</b>rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.</p><p><b>RESULTS</b>Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.</p><p><b>CONCLUSIONS</b>BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Fisiologia
/
Imuno-Histoquímica
/
Células Cultivadas
/
Fator de Crescimento Transformador beta
/
Consolidação da Fratura
/
Proteínas Morfogenéticas Ósseas
/
Proteína Morfogenética Óssea 2
/
Proteína Morfogenética Óssea 3
/
Fibroblastos
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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