Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 326-328, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-332940
ABSTRACT
<p><b>OBJECTIVE</b>To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellular allogenous nerve grafts (ANG) with chemical extraction.</p><p><b>METHODS</b>The double adhesion culture and Arab-c to prohibit the fibroblast growth were used to achieve high-purified Scs. Triton-x-100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was analysed.</p><p><b>RESULTS</b>High purified Scs and ANG were acquired, which could integrate each other well. Scs could survive and transfer to aline in vitro.</p><p><b>CONCLUSIONS</b>Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nervos Periféricos
/
Fisiologia
/
Ratos Endogâmicos F344
/
Células de Schwann
/
Nervo Isquiático
/
Cirurgia Geral
/
Transplante
/
Transplante Homólogo
/
Técnicas In Vitro
/
Imuno-Histoquímica
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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