nNOS expression of hippocampal neurons in aged rats after brain ischemia/reperfusion and its role in DND development / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 232-236, 2002.
Article
em En
| WPRIM
| ID: wpr-332961
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of neuronal nitric oxide synthase (nNOS) in aged rats' hippocampal delayed neuronal death (DND) following brain ischemia.</p><p><b>METHODS</b>Models of incomplete brain ischemia were induced by clipping common carotid artery. A total of 46 aged SD rats were divided into 8 groups: normal control group (Group A, n=5), sham-operation group (Group B, n=5), reperfusion 1, 6, 12, 24, 48, and 96 hours groups after brain ischemia for 30 minutes (Group C, D, E, F, G, and H, n=6/group). The expression of nNOS was examined by immunohistochemistry and neuronal ultrastructural changes were observed by the transmission electron microscopy (TEM) at different time points after reperfusion.</p><p><b>RESULTS</b>Immunohistochemistry showed that nNOS expression in the hippocampal neurons was high in Group E, low expression in Group D, moderate expression in Group F and G. There was nearly no expression of nNOS in Group A, B, C, and H. Ultrastructure of hippocampal neurons was damaged more severely in reperfusion over 24 hours groups.</p><p><b>CONCLUSIONS</b>Nitric oxide (NO) may be one of the important factors in inducing DND after ischemia/reperfusion.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Imuno-Histoquímica
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Microscopia Eletrônica
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Traumatismo por Reperfusão
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Isquemia Encefálica
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Ratos Sprague-Dawley
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Apoptose
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Óxido Nítrico Sintase
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Hipocampo
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Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2002
Tipo de documento:
Article