Effects of nerve growth factor on neuronal nitric oxide production after spinal cord injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 165-168, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-332975
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective effects of nerve growth factor (NGF) on injured spinal cord.</p><p><b>METHODS</b>The spinal cord injury (SCI) model of Wistar rats was established by a 10 gx2.5 cm impact force on the T(8) spinal cord. NGF (60 microg/20 microl) was given to the rats of the treatment group immediately and at 2, 4, 8, 12, 24 hours after SCI. The level of neuronal constitutive nitric oxide synthase (ncNOS) and the expression of ncNOS mRNA in the spinal cord were detected by the immunohistochemistry assay and in situ hybridization method.</p><p><b>RESULTS</b>Abnormal expression of ncNOS was detected in the spinal ventral horn motorneuron in injured rats. The levels of ncNOS protein in the NGF group were significantly lower than those in the normal saline group (P<0.05 ). The ncNOS mRNA expression was found in the spinal ventral horn motorneuron in injured rats and the expression in the NGF group was significantly decreased compared with that in the normal saline group (P<0.01).</p><p><b>CONCLUSIONS</b>NGF can protect the injured tissue of the spinal cord by prohibiting abnormal expression of nitric oxide synthase and the neurotoxicity of nitric oxide.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Traumatismos da Medula Espinal
/
Imuno-Histoquímica
/
Ratos Wistar
/
Hibridização In Situ
/
Óxido Nítrico Sintase
/
Fator de Crescimento Neural
/
Óxido Nítrico Sintase Tipo I
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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