Molecular biological survey of tick-born arboviruses in southern part of Xinjiang / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 325-330, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-333013
ABSTRACT
<p><b>BACKGROUND</b>To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.</p><p><b>METHOD</b>Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd(N)6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA.</p><p><b>RESULTS</b>All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primers derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments.</p><p><b>CONCLUSION</b>Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Arbovírus
/
Carrapatos
/
Virologia
/
China
/
Classificação
/
Vírus da Febre Hemorrágica da Crimeia-Congo
/
Doenças Transmitidas por Carrapatos
/
Análise de Sequência de DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS