Isolation and identification of arboviruses from mosquito pools in Yunnan Province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 319-324, 2005.
Article
em Zh
| WPRIM
| ID: wpr-333014
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To survey arboviruses in Yunnan province.</p><p><b>METHODS</b>Mosquitoes were collected from Yunnan Province in 2002 and 2004. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK cell line. The isolated strains and their molecular biological characteristics were identified by real-time PCR, reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent antibody technique.</p><p><b>RESULTS</b>Twelve strains of viruses producing CPE in BHK cells were isolated from 4810 mosquitoes. All the 12 isolates were identified to be Japanese encephalitis viruses. Genotype analysis showed the new virus (DL-0437 strain) belonged to genotype III.</p><p><b>CONCLUSION</b>Twelve strains of Japanese encephalitis viruses were isolated from mosquito pools collected in Yunnan. It was the first isolation of genotype III Japanese encephalitis viruses in Yunnan Province in recent years.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Arbovírus
/
Virologia
/
Linhagem Celular
/
China
/
Proteínas do Envelope Viral
/
Classificação
/
Análise de Sequência de DNA
/
Encefalite Japonesa
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Article