Experimental study on the inhibitory effect of Carboxymethyl Pachymaram on hepatitis B virus expression from transfected cells / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the anti-HBV activity of Carboxymethyl Pachymaram (CMP) on the culturing of 2.2.15 cell line.</p><p><b>METHODS</b>Concentrations of 20.0 g/L, 12.0 g/L, 6.0 g/L, 3.0 g/L, 1.5 g/L of CMP were used to evaluate its toxicity to the cell line and the inhibition rates of the secretion of HBsAg and HBeAg in the cultured 2.2.15 cell line.</p><p><b>RESULTS</b>Experiments showed that the mean half toxicity concentration of CMP for 2.2.15 cell line was 13.6 g/L and concentration for 50% inhibition of the secretion of HBsAg and HBeAg were 4.45 g/L, 5.61 g/L and TI were 3.06 and 2.42. CMP showed stronger effect on anti-HBV than aciclovir.</p><p><b>CONCLUSION</b>CMP has good inhibitory effect on the secretion of HBsAg and HBeAg on cultured cell line 2.2.15.</p>