Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 80-83, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-333042
ABSTRACT
<p><b>OBJECTIVE</b>To design and rapidly evaluate a TaqMan assay for detecting influenza A viruses.</p><p><b>METHODS</b>The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0. Their specificity and conservation were verified through Blast in GenBank and electronic hybridization. The assay's sensitivity was compared with the standard RT-PCR.</p><p><b>RESULTS</b>The designed primers and probe were confirmed to be very specific and conserved. The assay was 3-27 folds more sensitive than the standard RT-PCR. The RT and PCR steps could be simplified into one step.</p><p><b>CONCLUSION</b>The TaqMan Real-time PCR assay is specific, sensitive and easy to perform.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Influenza A
/
Virologia
/
Aves
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Diagnóstico
/
Influenza Humana
/
Influenza Aviária
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Artigo
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