Establishment and application of fluorescent quantitative PCR method for detecting human herpes virus type 6 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 74-76, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-333044
ABSTRACT
<p><b>OBJECTIVE</b>To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6).</p><p><b>METHODS</b>According to the specific sequence of human herpes virus type 6 genes, the primers and the fluorescent probe (TaqMan) were designed and synthesized. The fragment generated from HHV 6 gene as template was cloned into the pMD18-T vector which was constructed from the pUC 18. And the positive recombinant plasmid was 110 diluted and used as standard quantitative template to make the standard curve for sample detection.</p><p><b>RESULTS</b>The standard curve indicated the linear relationship between Ct (cycle threshold) and template concentration. The clinical samples from 135 cases were detected by this system, 16 cases among 135 were positive.</p><p><b>CONCLUSION</b>The fluorescent quantitative PCR method for the detection of human herpes virus type 6 is simple and accurate, and this method may be helpful to clinical diagnosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
DNA Viral
/
Sondas de DNA
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Púrpura Trombocitopênica Idiopática
/
Primers do DNA
/
Herpesvirus Humano 6
/
Infecções por Roseolovirus
Tipo de estudo:
Estudo diagnóstico
Limite:
Adolescente
/
Adulto
/
Idoso
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Artigo
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