Identification of oligopeptides mimicking the virus attachment protein of hantaan virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 58-60, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-333049
ABSTRACT
<p><b>OBJECTIVE</b>To identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus.</p><p><b>METHODS</b>The monoclonal antibody 3G1 was used as the ligand to biospan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid sequences of them were compared with that of hantaan virus G2 in homology. The characteristics of positive phage were studied by IFA and ELISA. A decapeptide combining to cell membrane was observed under laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>The conservative motif PX(1-2) HX(0-2) H displaying on positive clones shared homologous amino acid sequence with G2 96YPWHTAKCHY105.</p><p><b>CONCLUSION</b>G2 96YPWHTAKCHY105 might play some roles in virus binding to host cell, and might be a possible key epitope of hantaan virus VAP.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligopeptídeos
/
Proteínas Virais
/
Células Vero
/
Ensaio de Imunoadsorção Enzimática
/
Membrana Celular
/
Chlorocebus aethiops
/
Cricetulus
/
Células CHO
/
Vírus Hantaan
/
Microscopia Confocal
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Artigo
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