In vitro infection of human hepatoma (Hep G2) cell line by hepatitis B virus positive serum / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 169-171, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-333071
ABSTRACT
<p><b>OBJECTIVE</b>To establish a culture system of HBV positive serum infected Hep G2 cells in vitro.</p><p><b>METHODS</b>Hep G2 cells were seeded into six-well cluster dishes, at 1 x 10(-6) cells per well and incubated with 3 ml 10% fetal calf serum/ Dulbecco's modified Eagle's medium (10% FCS/DMEM) at 37 degrees in 5% CO2 air. At 24 h after plating, infection group Hep G2 cells were cultured with 0.5 ml HBV positive serum, in control group HBV negative serum was used, 24 h later the inoculums was removed. The cells were then extensively washed with 0.01 mol/L phosphate-buffered saline (PBS). After washing with PBS, 4 ml 2% FCS/DMEM were added to each well and the medium was collected every 12 h. ELISA method was used to detect HBsAg in culture medium. HBV DNA in cells and culture medium was detected by PCR.</p><p><b>RESULTS</b>In infection group, HBsAg could be detected from cell culture medium from 12 h (after PBS washed) to 84 h. HBV DNA could be detected by PCR in culture medium and cells.</p><p><b>CONCLUSION</b>Infection of Hep G2 cells by HBV positive serum is feasible.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Virologia
/
Sangue
/
DNA Viral
/
Ensaio de Imunoadsorção Enzimática
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Carcinoma Hepatocelular
/
Linhagem Celular Tumoral
/
Soro
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS