Conditions for SARS-CoV cultivation and inactivation / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 135-137, 2005.
Article
em Zh
| WPRIM
| ID: wpr-333081
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the method for cultivation and inactivation of SARS-CoV.</p><p><b>METHODS</b>In order to choose the sensitive cell strain and the best infection dose of the virus, Vero, Vero-E6 and 2BS cell lines were infected with SARS-CoV. The cultivation temperature was selected among 25 degrees C, 33 degrees C and 37 degrees C. The best inactivating time and effect were observed with beta-propiolactone whose concentration ranged from 1:2000 to 1:20,000 at room temperature.</p><p><b>RESULTS</b>Vero and Vero-E6 cell lines were sensitive to SARS-CoV. The cytopathic changes of the cells were 75% at 37 degrees C in 5 percent CO2 incubator after infection. SARS-CoV was inactivated completely in beta-propiolactone (1:4000). The toxicity of beta-propiolactone was hydrolyzed completely when the inactivated virus was cultured for 16 hours at 2 degrees C, 8 degrees C and in water bath for 2 hours at 37 degrees C.</p><p><b>CONCLUSION</b>The titer of SARS-CoV was the highest when it was cultured in Vero or Vero-E6 cells for 72 hours at 37 degrees C in 5 percent CO2 incubator. SARS-CoV was inactivated completely in beta-propiolactone when its concentration was 1:4000 and the interaction time was 1 hour.</p>
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Propiolactona
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Temperatura
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Fatores de Tempo
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Células Vero
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Chlorocebus aethiops
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Inativação de Vírus
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Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
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Relação Dose-Resposta a Droga
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2005
Tipo de documento:
Article