Your browser doesn't support javascript.
loading
Influence of the mutants of hepatitis B surface antigen on the cell immunity / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 124-127, 2005.
Artigo em Chinês | WPRIM | ID: wpr-333084
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of the mutants of hepatitis B surface antigen on the cell immunity.</p><p><b>METHODS</b>The recombinant plasmids of NS2Swt, NS2S126, NS2S133, NS2S141 and NS2S145 were transfected into Chinese hamster ovary (CHO) cells and the expressed proteins were detected by means of ELISA. Following PHA-activated lymphoblasts proliferation assay and IFN-gamma, IL-2, IL-10 induction assay were done with these proteins.</p><p><b>RESULTS</b>It was identified that these proteins of HBsAg could stimulate human lymphoblasts proliferation. Besides, there were no significant difference between the mutants and the wild. It was deserved to point out that the HBsAg with T126S mutation could increase the expression of IFN-gamma in the culture medium while the HBsAg with M133T mutation induced more expression of IL-10.</p><p><b>CONCLUSION</b>The results suggested that the cellular immune response to mutants of HBV might not be strengthened or weakened. But it should not be ignored that HBV T126S or M133T mutation may assert a potential impact on the cell immunity.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Plasmídeos / Proteínas Recombinantes / Ensaio de Imunoadsorção Enzimática / Linfócitos / Transfecção / Células Cultivadas / Cricetulus / Interferon gama / Interleucina-2 Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2005 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Plasmídeos / Proteínas Recombinantes / Ensaio de Imunoadsorção Enzimática / Linfócitos / Transfecção / Células Cultivadas / Cricetulus / Interferon gama / Interleucina-2 Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2005 Tipo de documento: Artigo