Gene chip analysis of mutation of HBV-DNA open reading frame / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.</p><p><b>METHODS</b>By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.</p><p><b>RESULTS</b>The mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.</p><p><b>CONCLUSION</b>The gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.</p>