Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells / 生理学报
Acta Physiologica Sinica
;
(6): 444-448, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-333181
ABSTRACT
The aim of the present study was to investigate the effect of glucagon-like peptide-1 (GLP-1) on palmitate-induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the underlying mechanism. HUVECs were cultured in vitro, and then treated by palmitate to induce apoptosis. Meanwhile, GLP-1 was added to explore its effect. After 24 h of the treatments, Caspase-3 activity and DNA fragmentation were measured using ELISA kits. Phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) expression was detected by Western blot. The results showed that incubating HUVECs with 0.125 mmol/L GLP-1 increased Caspase-3 activity and DNA fragmentation. GLP-1 significantly inhibited palmitate-induced increases of Caspase-3 activity and DNA fragmentation in a concentration-dependent manner. Moreover, GLP-1 inhibited the up-regulation of p-p38 MAPK expression induced by palmitate in HUVECs. These results suggest GLP-1 protects HUVECs against lipo-apoptosis, and this effect may be mediated through inhibiting p38 MAPK pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Regulação para Cima
/
Apoptose
/
Sistema de Sinalização das MAP Quinases
/
Biologia Celular
/
Proteínas Quinases p38 Ativadas por Mitógeno
/
Peptídeo 1 Semelhante ao Glucagon
/
Caspase 3
/
Fragmentação do DNA
/
Células Endoteliais da Veia Umbilical Humana
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2012
Tipo de documento:
Artigo
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