Effect of Epstein-Barr virus-encoded latent membrane protein 1 on β-catenin transcriptional activity and expression in nasopharyngeal carcinoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 537-541, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-333257
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the contribution of latent membrane protein (LMP)1 to nasopharyngeal carcinogenesis via Wnt/β-catenin signal pathway.</p><p><b>METHODS</b>The recombinant plasmid pHA2-LMP1 was constructed; immunofluorescence staining, Dual-Luciferase Reporter Assay, Western blot and immunohistochemistry staining were used to study the effect of LMP1 on the transcriptional activity and expression of β-catenin.</p><p><b>RESULTS</b>(1) Abnormal expression of β-catenin was obtained in 38 cases (50.7%, 38/75), LMP1 expression was obtained in 38 cases (50.7%, 38/75). There was significantly positive correlation between LMP1 expression and abnormal expression of β-catenin in nasopharyngeal carcinoma tissue (P = 0.008). (2) The expression of β-catenin in nuclei of NPC cell line CNE1 and CNE2 transfected with pHA2-LMP1 plasmid dramatically increased, and the expression was remarkable in poorly-differentiated NPC cell line CNE2 than that of well-differentiated CNE1 cells. (3) LMP1 expression dramatically increased the transcriptional activity of β-catenin in CNE1 and CNE2 cells transfected with pHA2-LMP1 and was in a time-dependent. The transcriptional activity of β-catenin was higher in poorly-defferentiated cell line CNE2 than that of well-differentiated NPC cell line CNE1. (4) LMP1 expression did not affect the total protein expression level of β-catenin in both CNE1 and CNE2 cell lines.</p><p><b>CONCLUSION</b>EB virus-encoded LMP1 may be involved in the pathogenesis of NPC via β-catenin signal pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Plasmídeos
/
Proteínas Recombinantes
/
Transfecção
/
Transdução de Sinais
/
Regulação Neoplásica da Expressão Gênica
/
Ativação Transcricional
/
Proteínas da Matriz Viral
/
Neoplasias Nasofaríngeas
/
Linhagem Celular Tumoral
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2010
Tipo de documento:
Artigo
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