Effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 368-372, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-333317
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells (HPDLC).</p><p><b>METHODS</b>HPDLC were cultured on flexible-bottomed plates and subjected to 12% elongation by strain unit at 6 cycles/min (i.e.5-s elongation and 5-s relaxation) for 48 hours in the experimental groups. GEArray Q series Human Osteogenesis Gene Array was used to identify the genes expressed in HPDLC, including growth factors and associated molecules, extracellular matrix and its associated proteins, cell adhesion molecules and housekeeping genes. The changes in the expression of 96 representative transcripts were determined by arrayed cDNA hybridization.</p><p><b>RESULTS</b>After application of tension force, 21 genes were significantly upregulated, including 10 growth factors and associated molecules, 10 extracellular matrix and its associated protein and 1 cell adhesion molecules. Two genes were significantly downregulated, including 1 growth factors and associated molecules and 1 cell adhesion molecules.</p><p><b>CONCLUSIONS</b>The HPDLC can differentiate into osteoblast-like cells by mechanical stretch induction through the activation of some osteogenesis genes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Ligamento Periodontal
/
Estresse Mecânico
/
Moléculas de Adesão Celular
/
Diferenciação Celular
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Criança
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
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