Increased expression of serum gelsolin in patients with osteosarcoma / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 262-269, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-333504
ABSTRACT
<p><b>BACKGROUND</b>Identification of potential serum biomarkers of osteosarcoma to aid in its early diagnosis and in the discovery of possible therapeutic targets is an area of increasing interest.</p><p><b>METHODS</b>Two-dimensional difference-in-gel electrophoresis was used to assess multiple serum samples in patients with osteosarcoma. In addition, differential expression of protein biomarkers was characterized in osteosarcoma serum by using matrix-assisted desorption/ionization time-of-flight mass spectrometry coupled with database interrogation. Serum samples from four individuals with osteosarcoma and four age- and sex-matched healthy control subjects were compared.</p><p><b>RESULTS</b>Fifty-eight significant protein spot features in the osteosarcoma sera were found. These spot features were excised, digested with trypsin, and analyzed with mass spectrometry. Gelsolin was down-regulated only in osteosarcoma. Furthermore, Western blotting and enzyme linked immunosorbent assay (ELISA) confirmed decreased levels of gelsolin in the osteosarcoma serum samples.</p><p><b>CONCLUSIONS</b>These results indicated that gelsolin might have great potential as a biomarker of osteosarcoma and as a potential target for gene therapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Ensaio de Imunoadsorção Enzimática
/
Eletroforese em Gel Bidimensional
/
Biomarcadores Tumorais
/
Osteossarcoma
/
Western Blotting
/
Gelsolina
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Tipo de estudo:
Estudo de rastreamento
Limite:
Adolescente
/
Adulto
/
Criança
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2012
Tipo de documento:
Artigo
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